This study was undertaken to determine the effects of VernoniaAmygdalinaon Phenyl hydrazine-induced kidney damage in rats.
Methodology:- Thirty (30) Albino rats were randomly divided into five groups of six rats each (n=6).
Group 1 was normally controlled and the rats were fed with standard rat pellets diets and water. Groups 2-5 were the experimental group and the rats were treated with an equal dose of phenylhydrazine.
Group 2 consist of phenylhydrazine-induced kidney damage; the rats were treated with phenylhydrazine only.
Group 3 was administered 500 mg/kg of the extract simultaneously with phenylhydrazine.
Groups 2-3 were sacrificed under chloroform vapour on the fourth day in other to ensure that the level of phenylhydrazine is enough to induce renal damage.
Group 4 was post-treated with 400 mg/kg of the extract for 14 days. Group 5 was post-treated with 300 mg/kg of the extract for 28 days.
Groups 4-5 were then euthanized under chloroform vapour and were sacrificed immediately after the respective final day of administration. The kidney was surgically removed, immediately blotted using filter paper to remove traces of blood, weighed with an analytical balance then fixed in 10% formal saline preparatory to histological processing.
Results:-The experimental animals treated with phenylhydrazine only were observed to produce body weakness, loss of appetite, a decline in body weight, and respiratory distress.
Conclusion:- The results suggest that Vernoniaamygdalina (bitter leaf) have a great level of renal recovery from phenylhydrazine-induced kidney damage. It has nephroprotective effects; it is, therefore, safe to consume so as to enhance renal functioning. Further research should be carried out in humans because the renal disease is a problem now globally.
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